CELLZSCOPE +

NanoAnalytics

CELLZSCOPE +

The cellZscope+ measures the impedance of barrier-forming cell cultures grown on permeable membranes under physiological conditions. Main features are:

  • Easy readout parameters: TER and Ccl of the cell layer
  • Full compatibilty with a broad range of standard cell culture inserts
  • Cell Module can be loaded with 24 inserts simultaneously

Technical Note
Application Note

Description

The cellZscope+ is computer-controlled and allows automated fast process monitoring as well as reliable long term measurements over days and weeks. The ohmic resistance (TER, transepithelial / -endothelial resistance) and capacitance (Ccl) of the cell layers under investigation are determined in real-time.

The convincing performance and instrument operations and easy maintenance make the cellZscope+ the ideal tool for controlling cellular barriers. It sweeps over a wide frequency range instead of simply measuring at a very few frequency points. This ensures a reliable and unambiguous detection of cell layer properties.

cellZscope - Hardware

The cellZscope+ is designed to ensure simple operation and maximum flexibility. Its Cell Module provides investigators full apical and basolateral access to all wells and is easily transferable to a laminar flow hood.

Single-piece stainless steel “pots” serve as bottom electrodes, as vessels for holding the cell culture medium, and as supports for cell culture inserts.

cellZscope - Hardware

A magnetic mount makes handling of the upper electrodes comfortable and accurate positioning is guaranteed. Top and bottom electrodes can be detached easily without the use of tools. These features make the cellZscope systems a snap to clean and sterilize.

cellZscope - Hardware

Three well sizes are available for use with small (“24-well” type), mid-sized (“12-well” type), or large (“6-well” type) inserts, respectively.

Different sizes of cell culture inserts can be combined within a single Cell Module. Thus allowing the automated monitoring and direct comparison of the barrier properties of cell layers housed in up to three different sized cell culture inserts.

cellZscope - well sizes

The cellZscope+ is compatible with a variety of standard cell culture inserts from different manufacturers. Three versions of the electrodes are available. They are tailored for use with small (“24-well” type), mid-sized (“12-well” type), or large (“6-well” type) inserts, respectively. Different well sizes can be combined within one Cell Module.

 

cellZscope - compatible inserts
Some examples of compatible cell culture inserts (nanoAnalytics GmbH assumes no responsibility or liability for any errors or inaccuracies).

 

Additional information

Dimensions (L×W×H)

Controller 26 cm × 6 cm × 27 cm (10.2 in × 2.4 in × 10.6 in)
Cell Module 32 cm × 24 cm × 7 cm (12.6 in × 9.5 in × 2.8 in)
Space in incubator 40 cm × 29 cm × 9 cm (15.8 in × 11.5 in × 2.8 in)

Connector Cable

Length 150 cm (59 in)
Cross section (W×T) 35 mm × 4 mm (1.4 in × 0.16 in)

*Usually the ribbon cable can be simply fed through the front door of the incubator.

Measurement Parameters

Frequency range 1 Hz to 100 kHz
Measurement time for 24 wells
– Standard resolution† ~ 18 min
– Reduced resolution‡ ~ 4 min
† 1 Hz – 100 kHz, fine frequency resolution
‡ 5 Hz – 100 kHz, coarse frequency resolution

Cell Module

Number of wells 24 (4 rows, 6 columns)
6-well size ✔
12-well size ✔
24-well size ✔

Minimum requirements for the control computer

– 1.0 GHz processor speed
– 1024 MB RAM
– USB 2.0 port
– 1024×768 display
– Microsoft Windows® 7/8/10

Power Supply

100 – 240 Vac; 0.8 A; 47 – 63 Hz